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Selective Platelet Activation by Surfaces and Soluble Agonists

Lundi 20 janvier 2014 15:00 - Duree : 1 heure
Lieu : EMBL seminar room, ILL - 6 rue Jules Horowitz - Grenoble

Orateur : Ilya REVIAKINE (Institute for Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT) Germany)

Platelets are anuclear 3 – 4 ìm cell fragments circulating in the blood. They are a key component of the complex coagulation machinery that evolved to limit traumatic blood loss in case of injury, but that misfires under pathological conditions such as atherosclerosis leading to thrombotic complications that cause heart attacks and strokes. Recent evidence suggests that platelets are furthermore involved in numerous other physiological and pathological processes such as wound healing, immune response, cancer metastasis, angiogenesis, and vascular remodeling. Platelets circulate in a resting state but get activated at the site of injury to become procoagulant, catalyze clot formation, and release active substances stored in their granules. The diversity of platelet functions implies that their responses are regulated, tailored to fulfilling particular functions under specific physiological conditions. Selective regulation of platelet responses is intensely studied because of its therapeutic potential. We investigated platelet activation by surfaces of biomaterials and by soluble agonists, and found evidence of such selective responses. In particular, platelets expressed different sets of markers when adhering on glass vs. TiO2 in the absence of extracellular calcium. This difference in marker expression correlated with different intracellular calcium dynamics in platelets adhering on the two surfaces, implying that different signaling pathways were involved. A common approach to studying platelet activation is to use antibodies against surface glycoproteins. However, this approach has limitations. Instead, we chose to examine how platelet surface glycosylation changed in response to soluble agonists. For this purpose, we used fluorescently labeled lectins (carbohydrate binding proteins). Using lectins, it was possible to distinguish platelets activated with different soluble agonists, from each other, because each agonist led to a particular lectin-binding fingerprint.

Contact : campbell@ill.eu

Discipline évènement : (Physique)
Entité organisatrice : (ILL)
Nature évènement : (Séminaire)
Evènement répétitif : (General ILL Seminar - College 9)
Site de l'évènement : Polygone scientifique

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