Liquid-Liquid Phase Separation and Intermolecular Interactions in Dense Protein Solutions –High pressure SAXS studies on lysozyme solutions of high ionic strength
Mardi 25 mars 2014 15:00
- Duree : 1 heure
Lieu : Room 500 - 501, Central Building, ESRF - 6 Rue Jules Horowitz - Grenoble
Orateur : Johannes MOLLER (Technische Universitaet Dortmund, Allemagne)
Highly concentrated protein solution were found to yield a large number of different phases, like amorphous aggregates, gels, crystals, or a liquid-liquid phase separation, which are all governed by the underlying protein-protein interactions in water. The interactions present in dense protein solutions are highly complex, changes in the aqueous environment influence the inter- and intramolecular interactions, the protein-solvent interactions, and the solvent-solvent interactions. Small angle X-ray scattering in combination with hydrostatic pressure perturbations was used to investigate the intermolecular interaction potential and the resulting phase behavior of dense lysozyme solutions. A reentrant liquid-liquid phase separation at elevated pressures was discovered, which we could connect to the solvent mediated protein-protein interactions.
Contact : eva.jahn@esrf.fr
Prévenir un ami par email
Télécharger dans mon agenda