State-of-the-art analysis of SAS data

Orateur : Dmitri SVERGUN (European Molecular Biology Laboratory, Hamburg Outstation, EMBL c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany)

The tremendous progress in the instrumentation and analysis methods made small-angle scattering of X-rays and neutrons (SAXS/SANS) an extremely powerful technique addressing increasingly complicated questions in structural biology. The development of analysis techniques follows two major needs of modern SAXS/SANS. First, the huge amounts of data collected in robotic high throughput mode and with in-line purification devices at modern stations, call for a full automation of the data processing, extraction of the overall parameters and preliminary shapes from the experimental data. Second, advanced methods are required to not only reconstruct particle shapes ab initio but also to adequately explore the synergy of SAXS/SANS with other structural and biophysical methods. Scattering techniques play a major role in hybrid applications, where structural models are constructed using complementary low and high resolution information. Recent developments in the automated analysis and in the advanced SAS-based hybrid methods will be presented and illustrated by examples of practical applications.

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