When NMR spectroscopy is not enough : applications of ß-radiation detected NMR in chemistry and biochemistry

Orateur : Monika STACHURA (TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada)

Some biologically highly relevant metal ions, such as Mg(II), Cu(I) and Zn(II), are silent in most spectroscopic techniques leaving wide gaps in understanding their function in different physiological processes. Recently ß-radiation detected NMR (betaNMR) spectroscopy was successfully applied to liquid samples at ISOLDE-CERN, Switzerland, and ISAC-TRIUMF, Canada. The 31Mg-NMR signal recorded for Mg ions implanted into a drop of an ionic liquid (EMIM-OAc with MgCl2) showed two clear resonances which originate from Mg(II) occupying two different coordination geometries. This result demonstrates that betaNMR spectroscopy can be used to study the nuclear magnetic (and quadrupolar) interactions in different Mg(II) compounds/complexes making the first and very important step towards the application of this technique into biological systems, especially where low metal ion concentrations and biologically-relevant environments are important. This experiment also demonstrates new opportunities for carrying out NMR experiments on chemical elements that are currently not suitable for conventional NMR spectroscopy, such as Mg(II), Cu(I) or Zn(II).

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