Applications of antibody phage display in materials science and directed evolution
Mercredi 10 juillet 2013 11:00
- Duree : 1 heure
Lieu : CIBB seminar room, second floor, ILL - 6 rue Jules Horowitz - Grenoble
Orateur : Clément NIZAK (ESPCI, Paris)
Phage display is a powerful technique to perform quantitative selection from large libraries (typically 109 variants) of DNA sequences encoding proteins/peptides bearing a function of interest. We select antibody-encoding DNA sequences from artificial immune repertoires (antibody phage display) to generate antibodies binding various non-biological (polymer, metal, semi-conductor) targets. Sequence analysis of the selected antibody pool yields new insights into the interactions of antibodies and proteins with these non-biological molecules and surfaces. Selected antibodies are then engineered for applications such as animal imaging and self-assembly of new materials. Finally, I will explain how this selection technique can be coupled to controlled in vitro mutagenesis (affinity maturation) to perform quantitative molecular evolution.
Contact : sultan@ill.fr
Discipline évènement : (Physique)
Entité organisatrice : (ILL)
Nature évènement : (Séminaire)
Evènement répétitif : (General ILL Seminar - College 8)
Site de l'évènement : Polygone scientifique
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