A proton wire to couple substrate accommodation and peptide bond formation on the ribosome

Orateur : Axel INNIS (Institut européen de chimie et biologie, Pessac)

Peptide bond formation takes place on the large ribosomal subunit within an active site composed primarily of RNA. Although the ribosome’s main role in catalysis is to decrease the entropy of activation for the reaction, the lack of high-resolution structures of the complete ribosomal active site with bound full-length substrates/products has made it difficult to assess its role in coordinating the movement of protons during catalysis. Here, I will discuss recent crystal structures of pre-attack and post-catalysis complexes of the bacterial ribosome that suggest an alternative mechanism for peptide bond formation.

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