Structural dynamics of single metabotropic glutamate receptors dimer

Orateur : Emmanuel MARGEAT (Centre de Biochimie Structurale, Montpellier)

Metabotropic glutamate receptors (mGluR) are members of the class C G-protein Coupled Receptors (GPCR) family. They are activated by glutamate, the major excitatory neurotransmitter in the central nervous system. In this presentation, I will present how we used single molecule Förster Resonance Energy Transfer (smFRET) associated with advanced data analysis methods to measure at the microsecond timescale the conformational changes associated with the binding of various ligands to these receptors. We demonstrate that the receptor exists in a preformed equilibrium of conformational states and that the exclusive role of ligands, including partial agonists, is to modulate the transition rates between states, rather than stabilizing specific and unique conformations of the receptor.

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