Micro and nanotechnologies for life sciences : single molecule combing, microfluidics for neurosciences and infrared stimulation of action potential in neurons

Orateur : Benoît CHARLOT (Institut d'Electronique et des Systèmes, Univ. Montpellier)

This presentation will show several developments of bioMEMS and microfluidic devices for cell biology. The first part will present some developments of molecular combing techniques used for the deposition of single DNA fragments on micro and nano structured substrates. In order to visualize by fluorescence the number and position of replication sites, it is mandatory to be able to trap DNA fragments in uncoiled conformations. To do so, we use the molecular combing technique that uses the capillary forces that appear during the dewetting of a DNA solutions for the uncoiling and ordered deposition of DNA fragment in elongated conformations. Recent work using dewetting on nanogratings will be presented. The second part of the presentation will focus on the development of microstructured substrates and microfluidic cell culture devices for in-vitro neuronal junction reconstruction including a network of micro electrodes for the stimulation and recording of extracellular activities. Finally the last part of the presentation will show some research on direct stimulation of action potential in neurons by optical stimulation using infrared laser pulses.

Télécharger dans mon agenda