Exploring the structure and dynamics of multi-domain proteins : experimental and computational approaches
Vendredi 9 octobre 2015 11:00
- Duree : 1 heure
Lieu : Salle des séminaires de l’IBS - 71, avenue des Martyrs - Grenoble
Orateur : Cédric LEYRAT (The Division of Structural Biology, Oxford University)
In this talk I will discuss the power of hybrid approaches, combining experimental structural biology approaches such as X-ray crystallography and SAXS, with molecular dynamics simulations and
computational modeling, for characterizing the conformational dynamics of multi-domain proteins. I will discuss two examples of proteins displaying large conformational changes between functionally
distinct states. The M2-1 protein of human metapneumovirus (HMPV) is a zinc-binding transcription antiterminator which is highly conserved among pneumoviruses. The protein features a N-terminal zinc finger domain and an α-helical tetramerization motif forming a rigid unit, followed by a flexible linker and an α-helical core domain. The crystal structure of the asymmetric tetramer, molecular dynamics simulations and SAXS demonstrate a dynamic equilibrium between open and closed conformations in solution. Adseverin is a member of the calcium-regulated gelsolin superfamily of actin-binding proteins. Crystal structures, SAXS combined with ensemble optimization and molecular dynamics simulations provide new insights about a dynamic Ca2+-dependent equilibrium between inactive, intermediate and active conformations revealing structural similarities and differences with gelsolin.
Contact : ibs.seminaires@ibs.fr
Discipline évènement : (Biologie / Chimie)
Entité organisatrice : (IBS)
Nature évènement : (Séminaire)
Evènement répétitif : (Séminaire IBS)
Site de l'évènement : Polygone scientifique
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