A role for NOX enzymes in epileptogenesis ?
Mardi 10 novembre 2015 13:00
- Duree : 1 heure
Lieu : Amphithéâtre Serge Kampf, Grenoble Institut des Neurosciences (GIN) - Bât. Edmond J. Safra, Chemin Fortune Ferrini CHU, La Tronche
Orateur : Julia SIMON (équipe Depaulis)
The general aim of our study is to determine the changes of NADPH oxidases (NOX) and related genes pathways in Mesio Temporal Lobe Epilepsy (MTLE) to define possible therapeutic targets. MTLE is the most
common form of intractable focal epilepsy and only resective surgery is efficient to suppress seizures. In our laboratory, we developed a mouse model of MTLE (MTLE-KA mice) which consists in a single unilateral injection of kainate (KA) into the dorsal hippocampus of adult mice. This leads to the development of recurrent focal epileptic discharges within 2-3 weeks that stabilize afterwards, along with different features of hippocampal sclerosis (i.e., cell loss, gliosis and granule cell dispersions) also observed in human MTLE. Following transcriptomic analysis performed at different time-points after kainite injection (1, 3, 30 days), PU1 has been identified as the strongest candidate to regulate expression of altered NOX-related genes. Immunohistochemical studies confirmed the transcriptomic prediction of a long-term increase of PU1 expression in MTLE and its location in microglia.
Inhibition of NOX activity by apocynin treatment reduces NOX-related genes but does not affect KA-induced microglia activation neither impairs epileptogenesis in MTLE mouse.
Contact : yves.goldberg@ujf-grenoble.fr
Discipline évènement : (Biologie / Chimie)
Entité organisatrice : (GIN)
Nature évènement : (Séminaire)
Site de l'évènement : Pôle Santé / La Tronche
Prévenir un ami par email
Télécharger dans mon agenda