Cell-mediated fibre recruitment drives extracellular matrix mechanosensing in engineered fibrillar microenvironments
Mercredi 15 juin 2016 10:00
- Duree : 1 heure
Lieu : Grenoble INP-Phelma, Laboratoire LMGP-2ème étage-salle de séminaire, 3 parvis Louis Néel - 38000 Grenoble
Orateur : Brendon BAKER (Dpt of Biomedical Engineering, University of Michigan)
To investigate how cells sense stiffness in settings structurally similar to native extracellular matrices, we designed a synthetic fibrous material with tunable mechanics and user-defined architecture. In contrast to flat hydrogel surfaces, these fibrous materials recapitulated cell-matrix interactions observed with collagen matrices including stellate cell morphologies, cell-mediated realignment of fibres, and bulk contraction of the material. Increasing the stiffness of flat hydrogel
surfaces induced mesenchymal stem cell spreading and proliferation ; however, increasing fibre stiffness instead suppressed spreading and proliferation for certain network architectures. Lower fibre stiffness permitted active cellular forces to recruit nearby fibres, dynamically increasing ligand density at the cell surface and promoting the formation of focal adhesions and related
signalling. These studies demonstrate a departure from the well-described relationship between material stiffness and spreading established with hydrogel surfaces, and introduce fibre recruitment as a previously undescribed mechanism by which cells probe and respond to
mechanics in fibrillar matrices.
Contact : Michele.san-martin@grenoble-inp.fr
Discipline évènement : (Physique)
Entité organisatrice : (LMGP)
Nature évènement : (Séminaire)
Evènement répétitif : (Séminaire LMGP)
Site de l'évènement : Site Minatec
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