Lensfree video microscopy : high throughput monitoring and cell tracking of 2D and 3D cell cultures
Vendredi 23 septembre 2016 11:00
- Duree : 1 heure
Lieu : Salle des séminaires de l’IBS - 71, avenue des Martyrs - Grenoble
Orateur : Cédric ALLIER (CEA-LETI MINATEC, Département micro Technologies pour la Biologie et la Santé, Service Technologie pour la Détection)
By focusing on simplicity, cost, feasibility, field of view, and time-lapse in-incubator imaging, we developed a lensfree video microscope based on digital in-line holography. This system opens up new horizon to cell culture monitoring, by being able to capture the kinetics of thousands of cells simultaneously directly inside the incubators. And the resolution of the reconstructed image of 2 to 3μm yet allows to distinguish single cell and their micrometrics features : e.g. lamellipodia, pseudopodia, dendrites, etc. This permitted us to perform label-free cell based assays to study the major cellular events – cell adhesion and spreading, cell division, cell division orientation, and cell death. The results that we obtained respect the heterogeneity of the population, cell to cell variability (a raising concern in the biological community) and the massiveness of the population, whilst adhering to the standard cell culture practices. Most interestingly we have been able to measure at the single cell level the cell cycle duration and cell growth rate with an unprecedented statistics.
Contact : ibs.seminaires@ibs.fr
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