Cryo-electron microscopy in structural biology - revolution or evolution ?

Orateur : Helen SAIBIL (Professor of Structural Biology Crystallography, Dpt of Biological Sciences, Birkbeck College London)

Cryo-EM provides a powerful set of approaches to understanding the operation of macromolecular machines, both in isolation and in their cellular context. The methods have been developing over the last half-century in parallel with macromolecular crystallography, but lagging behind it by several decades. The recent major acceleration in the pace of development has brought cryo EM to prominence with a wider audience of structural and cell biologists, and the methods are also broadly applicable in materials science. In this lecture, I will cover the main principles and capabilities of cryo EM for molecular and cellular structure determination, and present examples of its application to the operation of macromolecular machinery, specifically molecular chaperones involved in protein folding, unfolding and disassembly of protein aggregates.

Télécharger dans mon agenda