ATTENTION !!!! SEMINAIRE ANNULE !!! Membrane protein production : new bacterial hosts in the T7 expression system
Vendredi 31 mars 2017 11:00
- Duree : 1 heure
Lieu : Salle des séminaires de l’IBS - 71, avenue des Martyrs - Grenoble
Orateur : Bruno MIROUX (Institut de Biologie Physico-Chimique, Paris)
Membrane proteins overproduction is one of the major bottleneck in structural genomics. Despite the emergence of eukaryotic expression system, bacteria remain a cost effective and powerful tool for protein production. The T7 RNA polymerase based expression system is the most successful and powerful system to achieve high level expression of membrane proteins. Several bacterial hosts and companion plasmids has been developed to tune the expression of the T7 RNA polymerase and avoid toxic effects associated with membrane protein production. However the lacUV5 promoter is difficult to repress and is poorly titrated by IPTG. Here we report a novel mechanism of regulation of the T7 expression system. Evaluation of newly isolated hosts with superfolder green fluorescent protein (sfGFP) and membrane proteins fused to GFP revealed an unprecedented tight control of the expression of the target gene resulting in a improved production yield and solubility of several bacterial and human membrane protein.
Contact : ibs.seminaires@ibs.fr
Discipline évènement : (Biologie / Chimie)
Entité organisatrice : (IBS)
Nature évènement : (Séminaire)
Evènement répétitif : (Séminaire IBS)
Site de l'évènement : Polygone scientifique
Prévenir un ami par email
Télécharger dans mon agenda