Dynamic remodeling of Dynamin complexes during membrane fission

Orateur : Tetsuya TAKEDA, Department of Biochemistry Okayama University Graduate School of Medicine, Japan

Dynamin is a mechanochemical GTPase essential for membrane fission in endocytosis. Dynamin forms helical complexes at the neck of endocytic pits and their structural changes upon GTP hydrolysis drive membrane fission. However, precise mechanisms of the Dynamin-mediated membrane fission remain to be elucidated. In this study, we analyzed dynamics of Dynamin complexes during membrane fission using electron microscopy and high speed-atomic force microscopy (HS-AFM). Interestingly, the Dynamin helices were dynamically remodeled to form clusters upon GTP hydrolysis and membrane fission occurred at the flanking “bare” membrane regions. Thus, our new approaches using HS-AFM demonstrated dynamics of Dynamin complexes during membrane fission for the first time proposing a novel mechanism of the Dynamin-mediated membrane fission.

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