Cell-free expression as a new alternative for difficult-to express proteins

Orateur : Bruno TILLIER (Synthelis, Grenoble)

Cell-free expression systems provide a real alternative for the expression of membrane proteins and other difficult-to-express proteins, enabling the study of their structure and function. In this presentation, we will first introduce the features and advantages of the cell-free expression systems. Then, we will present different case studies to illustrate our know-how and technology. Our core expertise is the implementation of the most suitable hydrophobic matrix for membrane protein production. This could be liposomes, nanodiscs or detergents to solubilize and stabilize membrane proteins during their expression and provide a format fitting to the final application. The proteins can then be used as antigen for immunization (vaccine or antibody development project), as target for screening and display technology, as material for structural biology, as reagent for IVD or as biologic for vectorization (protein therapy). Almost all target families have been successfully expressed in our system from mammalian targets (GPCRs, ion channels, etc…) to bacterial and viral proteins.

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