Mechanism of entry into cells of the cell penetrating peptide Penetratin

Orateur : Nicolas RODRIGUEZ (Laboratoire des BioMolécules, Sorbonne Université, Paris) - Hôte: Cécile Breyton (IBS/Groupe Membrane & Pathogènes)

Cell penetrating peptides share the ability to enter cells and can be used to vectorize cargos inside cells which makes them promising therapeutic agents. They can cross the plasma membrane through two classes of mechanisms : endocytosis and direct translocation. However the molecular details of these mechanisms and especially of the direct translocation are poorly understood. We adress this question through different experiments. First we have determined the role of the surface sugars on the plasma membrane during the first step of the adhesion of the peptide on the membrane through peptide-cell force measurements with the BioMembrane Force Probe technique. Secondly we have determined the influence of the lipid partners during the direct translocation of the peptide across model membranes formed at the interface between pairs of aqueous droplet in oil. Overall we have characterized the different steps of the peptide-membrane interaction and the different partners that favor the internalization of the peptide.

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