Selective Enlightenment of Interaction Surfaces in Biological Systems by DNP-enhanced NMR
Jeudi 4 octobre 2018 11:00
- Duree : 1 heure
Lieu : Science Building Room 036 - EPN campus - 71 avenue des Martyrs - Grenoble
Orateur : Ildefonso MARIN-MONTESINOSA (Univ. Grenoble-Alpes, CEA, CNRS, INAC-MEM, Grenoble, France)
X-ray crystallography, cryo-electron microscopy (cryo-EM) and nuclear magnetic resonance (NMR) are the main tools used in structural biology to solve the structure of proteins at atomic resolution. Beyond solving the structure of the proteins, it is also crucial to address interaction processes. This often remains a challenge, both in terms of selectivity and sensitivity for the aforementioned techniques.
Here we present a novel approach, dubbed SelDNP that enables the selective enlightenment of the residues located in the binding site of proteins using DNP-enhanced solid-state NMR. This powerful site-directed approach relies on the use of ligand-functionalized polarizing agents and is demonstrated on the binding site of the galactophilic lectin LecA. We report well resolved spectra, which enabled the de novo assignment of the binding interface residues. This approach allows resolving the main limitation of DNP-enhanced NMR for biomolecular applications, i.e. the lack of resolution often encountered at low temperature, while providing a new tool to address selectively binding interfaces.
Contact : mader@ill.fr
Discipline évènement : (Biologie / Chimie)
Entité organisatrice : (ILL)
Nature évènement : (Séminaire)
Evènement répétitif : (General ILL Seminar - College 8)
Site de l'évènement : Polygone scientifique
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