Directed Assembly of DNA through Flow, Confinement, and Condensation
Lundi 2 juillet 2012 14:00
- Duree : 1 heure
Lieu : Laboratoire Interdisciplinaire de Physique (ex LSP), salle 216 Bât. E, 140 Avenue de la Physique Domaine Universitaire, St-Martin-d’Hères
Orateur : Juan J de Pablo (Dept of Chemical and Biological Engineering, University of Wisconsin, Madison)
Beyond its importance to biology, the assembly of nanoscale objects on the basis of DNA hybridization is finding an increasing range of applications in materials science and engineering. In genetics, for example, the flow and translocation of long DNA molecules are of considerable applied and fundamental interest. In that context, design of effective genomic devices requires control of molecular shape and positioning at the level of microns and nanometers. In materials science, increasingly complex hierarchical structures can be created from DNA-coated nanoparticles through duplex formation. In that, context, understanding the rate of hybridization could lead to more effective strategies for synthesis of new materials.
This presentation will provide an overview of hierarchical models and computational approaches developed by our research group to investigate the effects of confinement, hydrodynamic interactions, and salt concentration, on the structure and properties of DNA, both at equilibrium and beyond equilibrium. A particular emphasis will be placed on emerging, detailed models and methods that are capable of describing melting and rehybridization at the single nucleotide level, as well as the packaging of DNA in chromatin. The validity of the models will be assessed by comparison to experimental data for dehybridization temperatures, molecular size and flexibility, rate constants for duplex formation, and nucleosome positioning.
Juan de Pablo est spécialiste de la modélisation des systèmes polymères nanostructurés, des verres et des fluides complexes. Il est professeur invité à l’UJF pour Juin et Juillet.
Website : http://www.engr.wisc.edu/che/faculty/depablo_juan.html
Discipline évènement : (Biologie / Chimie)
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Discipline évènement : (Physique)
Entité organisatrice : (LIPhy)
Nature évènement : (Séminaire)
Site de l'évènement : Domaine Universitaire de St Martin d’Hères
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