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Supramolecular photoactive systems for targeted antimicrobial photodynamic inactivation

Vendredi 1er décembre 2023 11:00 - Duree : 1 heure
Lieu : Salle de séminiare de l’IBS, 71 avenue des Martyrs, Grenoble

Orateur : Cristiano VIAPPIANI (Dipartimento di Scienze Matematiche, Università di Parma, Italy)

Résumé :

The use of photoactive molecules as light-controlled antimicrobial agents has been proposed as one of the possible strategies to tackle resistance insurgence to conventional treatments in microorganisms. Antimicrobial photodynamic inactivation (PDI) relies on the combined action of an otherwise nontoxic molecule (called photosensitizer), visible light, and oxygen to produce cytotoxic effects by the photoinduced generation of reactive oxygen species, particularly singlet oxygen.

Since light driven toxicity of the short-lived reactive oxygen species is a short range effect (in the order of 100 nm), one of the open issues is the development of delivery systems to enhance bioavailability and drive the photoactive compounds to the microorganism.

We have recently proposed supramolecular photoactive systems that exploit fully biocompatible and water soluble proteins to address the above problems. To achieve targeting, we proposed a modular approach where a photoactive protein (a modified streptavidin) is linked to a targeting protein, which is endowed with high affinity and molecular selectivity towards a molecular component located in the microorganism. Self-assembly of the building blocks is warranted by the strong streptavidin-biotin interaction.

Using a variety of spectroscopic and fluorescence imaging tools, we explore the effects of assembly on photo-functional properties of the photoactive assemblies, and demonstrate the capability of the supramolecular compounds in targeting and photoinactivating the microorganism.

References

1. Delcanale, P., et al., Subdiffraction localization of a nanostructured photosensitizer in bacterial cells. Scientific Reports, 2015. 5 : p. 15564.

2. Hally, C., et al., Photosensitizing proteins for antibacterial photodynamic inactivation. Translational Biophotonics, 2020. p. e201900031.

3. Mussini, A., et al., Versatile Supramolecular Complex for Targeted Antimicrobial Photodynamic Inactivation. Bioconjugate Chemistry, 2022. 33(4) : p. 666–676.

4. Delcanale, P., et al., The Interaction of Hypericin with SARS-CoV-2 Reveals a Multimodal Antiviral Activity. ACS Applied Materials & Interfaces, 2022. 14(12) : p. 14025-14032

5. Delcanale, P., S. Abbruzzetti, and C. Viappiani, Photodynamic treatment of pathogens. La Rivista del Nuovo Cimento, 2022. 405 : p. 407–459.

Contact : ibs.seminaires@ibs.fr

Discipline évènement : (Biologie / Chimie)
Entité organisatrice : (IBS)
Nature évènement : (Séminaire)
Evènement répétitif : (Séminaire IBS)
Site de l'évènement : Polygone scientifique

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